He is a vampire and stand user with the intent to kill the entire Joestar Bloodline, take over the world and achieve "Heaven" with his close friend Enrico Pucci, formerly kill George Joestar and take the Joestar fortune for himself.
His stand is The World, which allows him to stop the flow of time for 5 to 9 seconds, and with The World Over Heaven, rewrite reality.
in the SMG4 Universe, he is a retarded vampire who just likes to be an asshole and push around the Joestars and the Stardust Crusaders, most of the time being a creation of Inkweaver.
Dio appears in the television show Super Mario JoJo. He is seen confronting Jojo Mario and taunting him. Dio is then crushed by a road roller drove by Bob the Builder who is summoned by Jojo Mario, leaving him terrified.
- SMG4: World War Mario
- SMG4: Mario's Magical TV
- SMG4: Mario's Spicy Day 🔥
- SMG4: If Mario Was In... Anime
- His stand, The World, never appears in SMG4, however the Road Roller used by Dio in Stardust Crusaders is mentioned.
- Jojo Mario and Dio is an obvious reference to the final battle of Stardust Crusaders, the aftermath of Noriaki Kakyoin and Joseph Joestar's deaths, the latter of whom was brought back to life.
- He has a voice cameo in SMG4: Smart Mario