Dio Brando is a minor character in the SMG4 series.
Dio appears in the television show Super Mario JoJo. He is seen confronting Jojo Mario and taunting him. Dio is then crushed by a road roller drove by Bob the Builder who is summoned by Jojo Mario, leaving him terrified.
- His stand, The World, never appears in SMG4, however the Road Roller used by Dio in Stardust Crusaders is mentioned.
- Jojo Mario and Dio is an obvious reference to the final battle of Stardust Crusaders, the aftermath of Noriaki Kakyoin and Joseph Joestar's deaths, the latter of whom was brought back to life.
- He has a voice cameo in SMG4: Smart Mario