Honey Queen is a character from Super Mario Galaxy 1 and 2. She also appears in Mario Kart 7 as a playable character. In SMG4's videos, she only appears in R64: Stupid Mario Galaxy as the ruler of the Honeyhive Galaxy. She and her Bees worship Barry B. Benson. When she noticed Mario attack her bees then her by accident, she then ordered the bees to attack him back.
She later on returns in SMG4: Goodbye, SMG4 as Mario rams into her body twice, once while using the Warp Star while making games, the second time when Mario escaped Nintendo headquarters.