Mario's Tower is the setting of the episode Mario's Challenge.
During the challenge, Mario would guide the contestants through the floors. During each floor, some of the contestants would end up being eliminated.
Mario was waiting for the challengers at the top inside a tank. The only character to reach the top of the tower was Shroomy, though he was not necessarily the only one to survive the challenge.
- Frankie (crushed by gargoyle)
- E. Gadd (hit by a chair)
- Meggy (survived the challenge)
- Luigi (survived the challenge)
- Bowser (survived the challenge)
- Toad (loser)
- Toadette (fell into lava)
- Peach Toadstool (fell into lava)
- Fishy Boopkins (caught by one of the monsters living inside the maze)
- Bob Bobowski (crushed by gargoyle)
- Dr. Pootis (fell into lava)
- Wario (shot by a sentry gun that shot coins)
- Waluigi (struck down by Donkey Kong)
- Gourmet Guy (fell into a pit)
- Piranha Plant (hit by a meteor shower)
- Steve (Decapitated)
- Link (hit by a train)
- SMG3 (stomped by Bowser)
- Shroomy (Winner)
- Two additional floors appeared in Mario's Extras.
- It is unknown what happened to Mario's Tower following the challenge.