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Tuxie is a baby penguin found throughout Super Mario 64 in Cool, Cool Mountain Her Mother lost her and requires Mario to find her in exchange for a Star. In SMG4's videos, Tuxie is normally seen when Mario or someone is at Cool Cool Mountain. Mario almost always throws Tuxie off the edge like many players do.
In her latest appearance, it is revealed that she was actually a serial killer. She is currently in jail.