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Wario's mother looks just like Wario, but sports pigtailed anime hair.
Wario's mother first appears in a flashback by Wario, where she and her husband berate him for being so pathetic.
As Wario, in the form of the Wario Head, tries attack SMG4, the Mario Head reminds him that "the accident was thirty years ago", and that he has to let his mother go. Upon hearing this, Wario has a flashback to the moment his mother died, causing him to burst into tears.